Cell sorting is a process of physical separation of particles based on the differential fluorescence expression of one or more molecular antigens. Particle sorting allows biochemical, genetic, immunological, or cell differentiation and death assays to be performed on a specific group of cells or subcellular particles. Cell Sorters have an analyser that functions as a flow cytometer and a particle separator. The "Sorter" equipment has a Cytometer equipped with an optical configuration of 5 lasers, expandable to 6, which allows the characterisation and separation of the different biological elements on the basis of 20 parameters (18 fluorescences). The equipment is capable of simultaneously separating 4 subpopulations of particles under controlled temperature and agitation conditions. The separated populations can be collected in a one-cell-per-well format, allowing molecular studies of population heterogeneity and clonality. The minimum particle size that can be sorted is 100 nm, allowing the analysis of subcellular elements. The equipment is used to analyse the structural basis of antigen recognition, immune senescence, phenotypic heterogeneity in bacterial populations, metabolic reprogramming in cancer progression and neuronal degeneration.
Manufacturer: Becton Dickinson
Model: BD FACSAria Fusion 5 lasers
Location: Oncohematology Lab